automated luminex bead technologies

paa have had a long association with the automation the Luminex range of plate readers. The mode of action of the device is described elsewhere, Luminex multiplex bead plate readers

download app note Automated Luminex 100/200 with automate.it harmony download app note Bio-Rad Bio-Plex with a paa KiNEDx robot download app note Luminex 100 with a paa KiNEDx robot

categories high throughput screening forensic science applications high content screening automated luminex bead technologies Nanolitre dispensing automated surface chemistry automated atomic absorption Automation of ELISA assays dna science and technology automated hplc automated microbiology

The supplied systems have been built around Caliper (Zymark) Twister I and Twister II and Hamilton SWAP laboratory robots. These have been used to load the microplates with the treated beads via a barcode reader and a Thermo (H & P) shaker to the plate reader. The unique Overlord3 control method allows full control of this plate reader. These integrated Luminex plate readers have been integrated in a number of laboratories of major international pharmaceutical companies.

The basic automated Luminex system consists of:

• Luminex Plate Reader
• Robot - plate transport
• Plate stacks
• Shaker - to get the beads homogenised in the carrier fluid
• Barcode reader - to positively identify the plate

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Luminex with Twister I robot

The basic automated system has been expanded with:

• multiple Luminex readers in a single work cell
• Caliper Twister II, Hamilton SWAP, automate.it scara robots
• Tecan Gemini/EVO pipetting station for liquid handling before a plate read
• Bio-Rad Bio-Plex/ACS software control of the Luminex plate reader

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Luminex with Twister I robot

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Bio-Rad Bio-Plex with Twister I robot

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Luminex 100/ACS plate reader

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Luminex 100 with AB stacker

• unattended operation
• complete walk away functionality
• nearly 100% usage of the instrument
• no longer have to wait for instrument to complete the run
• plate tracking
• homogenisation of the plate contents before measurement
• warm up the instrument automatically read for use when you come in to the lab

The table below should help a user determine the available software for an automated workcell. All the instrument types below have been integrated into at least one system using Overlord scheduling software.

Applied Cytometry Systems (ACS) STarSTation software	Overlord3 SDK interface/Active X download app note Bio-Rad Bio-Plex with Twister I robot
Bio-Rad Bio-Plex software	Internal Overlord3 Control
Luminex Version 1.7 software	Internal Overlord3 Control
Luminex Version 2.2/2.3/3.x software	Luminex API